Culture Media for Bacteria
Salmonella species – Non Selective Media -Enrichment and Propagation
Strepto & Enterococcus
Campylobacter species -Enrichment Broth
Nutrient Broth is used for the general cultivation of less fastidious microorganisms , can be enriched with blood or other biological fluids.
Ingredients Gms / Litre
Sodium chloride 5.000
HM peptone B# 1.500
Yeast extract 1.500
Final pH ( at 25°C) 7.4±0.2
**Formula adjusted, standardized to suit performance parameters
Suspend 13.0 grams in 1000 ml distilled water. Heat, if necessary, to dissolve the medium completely. Dispense into tubes or flasks as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (6,7). Nutrient Broth has the formula originally designed for use in the Standard Method for Examination of Water and Waste water. It is one of the several non-selective media useful in routine cultivation of microorganisms (1,2). It can be used for the cultivation and enumeration of bacteria which are not particularly fastidious. Addition of different biological fluids such as horse or sheep blood, serum, egg yolk etc. makes it suitable for the cultivation of related fastidious organisms. Peptone, HM peptone B and yeast extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria. Sodium chloride maintains the osmotic equilibrium of the medium.
# – Equivalent to Beef extract
Type of specimen
Clinical samples – Blood ; Food and dairy samples ; Water samples.
Specimen Collection and Handling:
For clinical samples follow appropriate techniques for handling specimens as per established guidelines (6,7). For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (1,2,8). For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards.(3) After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
1. This medium is general purpose medium and may not support the growth of fastidious organisms.